Cell Culture:Article Title: Anti human Notch4 antibody
Article Snippet: .. Human non-small cell lung cancer cell line Calu6 (ATCC number HTB-56) cultured in an EMEM culture medium comprising 10% FBS, MEM non-essential amino acids, sodium pyruvate, and penicillin/streptomycin was prepared to a concentration of 1.6×108 cells/mL with EMEM culture medium, and mixed with MatrigelTM (CORNING Cat#354234) at 1:1 to prepare a cell suspension of 8.0×107 cells/mL. .. A dose of 0.1 mL was subcutaneously transplanted to the right posterior dorsal region of 6 weeks-old nude mice (CAnN.Cg-Foxn1nu/CrlCrlj, female, CHARLES RIVER LABORATORIES JAPAN, INC.) Twenty-eight days after transplantation, minor and major axes of the tumor were measured with an electronic digital caliper (DIGIMATICTM CALIPER, Mitsutoyo Corporation), and the tumor volume TV was calculated with the following calculation formula.
Article Title: Anti human Notch4 antibody
Article Snippet: .. Human non-small cell lung cancer cell line Calu6 (ATCC number HTB-56) cultured in an EMEM culture medium comprising 10% FBS and penicillin/streptomycin was prepared to a concentration of 1.2×108 cells/mL with EMEM culture medium, and mixed with MatrigelTM (CORNING Cat#354234) at 1:1 to prepare a cell suspension of 6.0×107 cells/mL. .. A dose of 0.1 mL was subcutaneously transplanted to the right posterior dorsal region of 7 weeks-old nude mice (CAnN.Cg-Foxn1nu/CrlCrlj, female, CHARLES RIVER LABORATORIES JAPAN, INC.) Twenty-seven days after transplantation, minor and major axes of the tumor were measured with an electronic digital caliper (DIGIMATICTM CALIPER, Mitsutoyo Corporation), and the tumor volume TV was calculated with the following calculation formula.
Concentration Assay:Article Title: Anti human Notch4 antibody
Article Snippet: .. Human non-small cell lung cancer cell line Calu6 (ATCC number HTB-56) cultured in an EMEM culture medium comprising 10% FBS, MEM non-essential amino acids, sodium pyruvate, and penicillin/streptomycin was prepared to a concentration of 1.6×108 cells/mL with EMEM culture medium, and mixed with MatrigelTM (CORNING Cat#354234) at 1:1 to prepare a cell suspension of 8.0×107 cells/mL. .. A dose of 0.1 mL was subcutaneously transplanted to the right posterior dorsal region of 6 weeks-old nude mice (CAnN.Cg-Foxn1nu/CrlCrlj, female, CHARLES RIVER LABORATORIES JAPAN, INC.) Twenty-eight days after transplantation, minor and major axes of the tumor were measured with an electronic digital caliper (DIGIMATICTM CALIPER, Mitsutoyo Corporation), and the tumor volume TV was calculated with the following calculation formula.
Article Title: Anti human Notch4 antibody
Article Snippet: .. Human non-small cell lung cancer cell line Calu6 (ATCC number HTB-56) cultured in an EMEM culture medium comprising 10% FBS and penicillin/streptomycin was prepared to a concentration of 1.2×108 cells/mL with EMEM culture medium, and mixed with MatrigelTM (CORNING Cat#354234) at 1:1 to prepare a cell suspension of 6.0×107 cells/mL. .. A dose of 0.1 mL was subcutaneously transplanted to the right posterior dorsal region of 7 weeks-old nude mice (CAnN.Cg-Foxn1nu/CrlCrlj, female, CHARLES RIVER LABORATORIES JAPAN, INC.) Twenty-seven days after transplantation, minor and major axes of the tumor were measured with an electronic digital caliper (DIGIMATICTM CALIPER, Mitsutoyo Corporation), and the tumor volume TV was calculated with the following calculation formula.
Suspension:Article Title: Anti human Notch4 antibody
Article Snippet: .. Human non-small cell lung cancer cell line Calu6 (ATCC number HTB-56) cultured in an EMEM culture medium comprising 10% FBS, MEM non-essential amino acids, sodium pyruvate, and penicillin/streptomycin was prepared to a concentration of 1.6×108 cells/mL with EMEM culture medium, and mixed with MatrigelTM (CORNING Cat#354234) at 1:1 to prepare a cell suspension of 8.0×107 cells/mL. .. A dose of 0.1 mL was subcutaneously transplanted to the right posterior dorsal region of 6 weeks-old nude mice (CAnN.Cg-Foxn1nu/CrlCrlj, female, CHARLES RIVER LABORATORIES JAPAN, INC.) Twenty-eight days after transplantation, minor and major axes of the tumor were measured with an electronic digital caliper (DIGIMATICTM CALIPER, Mitsutoyo Corporation), and the tumor volume TV was calculated with the following calculation formula.
Article Title: Anti human Notch4 antibody
Article Snippet: .. Human non-small cell lung cancer cell line Calu6 (ATCC number HTB-56) cultured in an EMEM culture medium comprising 10% FBS and penicillin/streptomycin was prepared to a concentration of 1.2×108 cells/mL with EMEM culture medium, and mixed with MatrigelTM (CORNING Cat#354234) at 1:1 to prepare a cell suspension of 6.0×107 cells/mL. .. A dose of 0.1 mL was subcutaneously transplanted to the right posterior dorsal region of 7 weeks-old nude mice (CAnN.Cg-Foxn1nu/CrlCrlj, female, CHARLES RIVER LABORATORIES JAPAN, INC.) Twenty-seven days after transplantation, minor and major axes of the tumor were measured with an electronic digital caliper (DIGIMATICTM CALIPER, Mitsutoyo Corporation), and the tumor volume TV was calculated with the following calculation formula.
|
ATCC is a verified supplier 
ATCC manufactures this product 